1800 244 735

Helpline (02) 9874 9777

Lost in translation? New insights into the making of the Huntington's disease protein

Everyone has two copies of the huntingtin gene but Huntington’s disease is caused by a copy that’s extra-long. New research shows that cells have different controls for how the normal and extra-long instructions are used to make protein. These controls on the protein-making process may be targets for developing drugs for HD.

You say potato…

We’ve known for twenty years now that the cause of Huntington’s disease is a mutation in the huntingtin gene. In people who develop the disease, one of the two copies has a repeated section that makes the gene extra-long.

By analogy, if we were to write the normal huntingtin gene as the word ‘potato’, patients with HD would have one copy of huntingtin misspelled as ‘potatato’ or even ‘potatatatato’.

It’s the extra-long copy of the huntingtin gene that makes neurons sick, because it causes them to produce an extra-long, harmful version of the huntingtin protein.

One problem facing scientists is to develop treatments that reduce the harm done by the extra-long protein, while preserving the useful functions of the normal-length protein. That’s not easy to do, because the proteins are identical except for the repeated section. However, some new research by a German team, published in the journal Nature Communications, has shed some new light on this problem.

The making of a protein

To understand this new research, first we’ll need to cover a few details about how cells actually make proteins.

The life of every protein starts off the same way, as a set of instructions written in the genetic code of the cell – our DNA. First, the cell makes a working copy of the DNA, made from a related chemical called RNA. This copying process is called transcription.

The RNA instructions float around the cell until they encounter a structure called a ribosome. When the instructions pair up with a ribosome, the ribosome uses them to assemble a protein. That process is called translation.

You can think of translation kind of like what happens when a chef prepares his world-famous chili: the chef (the ribosome) uses his favorite recipe (the RNA instructions) to make the chili (a protein).

Just like a chef sometimes has the help of an assistant chef when making his cuisine, ribosomes sometimes get a little bit of help making proteins. In these cases, the ribosomes join up with special helper complexes in the cell. The helper complex lets the ribosome translate genetic messages into protein more quickly than the ribosome could on its own.

A little help from my friends

With these details of translation in mind, a team of Huntington’s disease researchers, led by Susann Schweiger of The Max Planck Institute for Molecular Genetics in Berlin, decided to study how huntingtin proteins are made from genes of different lengths.

As expected, they found that the normal and extra-long genetic instructions were both translated into huntingtin proteins when they met up with a ribosome (the chef from our analogy above).

During translation, however, the RNA instructions for extra-long huntingtin can also interact with a helper complex (the assistant chef from our analogy). This interaction wasn’t seen with normal-length huntingtin RNA.

It turns out the the longer the RNA instructions are, the more they can interact with the helper complex. Because the helper complex makes translation more efficient, the result of this interaction was that the cells made more extra-length than normal-length huntingtin protein.

Lost in translation: reducing levels of extra-long huntingtin

The scientists wondered if they could affect levels of normal and extra-long huntingtin protein just by interfering with the helper complex.

Since the helper complex mostly interacts with the extra-length huntingtin instructions, interfering with it should reduce translation of the extra-long variety.

When the researchers blocked the helper complex using drugs, or prevented cells from making the helper complex in the first place, they got the result they expected – less of the extra-long huntingtin protein was made.

Decreasing extra-long huntingtin levels this way is appealing, because it happens before the protein ever gets made. If we could do it in people, it’d mean that extra-long or ‘mutant’ huntingtin would never even have a chance to make neurons sick.

Does this affect the search for HD treatments?

This research shows one effective way to alter production of normal and extra-long huntingtin selectively. It would be a form of ‘huntingtin lowering’ or ‘gene silencing’, but one that doesn’t reply on DNA-like or RNA-like drugs, which are difficult to deliver to the brain.

In addition to the helper complex itself, scientists are also looking at targets that work further down the protein production line.

One such target is mTOR – a protein that is actually already on the scene as a potential target in Huntington’s disease therapy.

We’ve known for a little while that drugs that interfere with mTOR decrease levels of mutant huntingtin protein by helping cells destroy it after it’s made. The new research shows that these drugs may pack a second punch, too, by decreasing the amount of extra-long huntingtin that gets made in the first place.

mTOR is particularly tantalizing as a drug target because the FDA, which regulates which drugs can be used in humans, has already approved some mTOR inhibitors for cancer treatment and in organ transplants. If interfering with mTOR really is an effective treatment strategy, drugs that already exist could be repurposed for use in Huntington’s disease.

Should we pop the champagne?

Not yet! First off, all of this new research on the role of the helper complex and mTOR in making extra-long huntingtin protein was done in cells or mice. These laboratory models are only the first step in understanding the human disease, so a lot of work remains before we’ll know if these pathways are actually important for real people with Huntington’s disease.

Second, even if these pathways are important, the other effects of drugs targeting these pathways may make it difficult to use them in Huntington’s disease.

For example, the FDA-approved mTOR inhibitors described above work because interfering with mTOR is toxic and suppresses the immune system. That makes the drugs effective against cancers and for preventing transplant rejection. But since mTOR inhibitors would need to be taken for many years in Huntington’s disease, these effects may make them unsuitable as HD therapies.

The bottom line

This work is a fascinating new approach that’s moving Huntington’s disease research in the right direction. The more we understand about how the normal and extra-long huntingtin proteins are made and work in brain cells, the better equipped we’ll be in the search for HD treatments.

Latest Research Articles

Roche Phase II GENERATION HD2 study underway

Published date: 14 February, 2023

Roche released a community letter last month, detailing how their Phase II clinical trial to study the huntingtin-lowering drug, tominersen, is now underway. Learn more about what this means in this article and at the recent HDSA Research Webinar, with representatives from the company. The ups and downs of huntingtin-lowering Tominersen is a type of ... Read more

Disappointing news from Novartis about branaplam and the VIBRANT-HD trial

Published date: 9 December, 2022

The pharmaceutical company Novartis has released a community update which announces that they are ending development of branaplam, a huntingtin lowering drug, for possible treatment in Huntington’s disease (HD). This news comes following recent bad news about side effects of branaplam in HD patients, being tested in the VIBRANT-HD clinical trial, dosing of which was ... Read more

Update on the PTC Therapeutics PIVOT-HD Trial

Published date: 2 November, 2022

Recruitment of participants into the US arm of the PTC Therapeutics PIVOT-HD trial has been paused. Since this announcement, there have been a lot of different (and confusing!) headlines about the pause in recruitment. In this article, we will lay out what is going on and what this announcement means. What is the aim of ... Read more

Forward momentum for Roche and Wave in latest news about huntingtin-lowering trials

Published date: 30 September, 2022

In the past week or so, during and following a big HD research conference, two companies developing medicines for Huntington’s disease announced news about their huntingtin-lowering drugs. First, the pharmaceutical company Roche announced plans for a new clinical trial of tominersen. Then, the genetic medicines company Wave Life Sciences shared early data showing that its ... Read more

Focusing in on fibrils; scientists give us a glimpse of huntingtin protein clumps

Published date: 8 September, 2022

A group of scientists from the EPFL in Lausanne, Switzerland have published a paper in the Journal of the American Chemical Society, describing clumps made up of a fragment of the huntingtin protein. A word that’s commonly used to describe these is “aggregates.” Using very powerful microscopes, the team was able to zoom in and ... Read more

Hereditary Disease Foundation (HDF) conference 2022 – Day 4

Published date: 2 September, 2022

DNA repair and CAG repeat instability The effect of HTT lowering on CAG repeat expansions Welcome to last day of the @hdfcures conference! We’ll only be sharing a few talks from today’s sessions, which focus on DNA repair. The first is from HDBuzz’s very own Jeff Carroll! Jeff will be sharing his work on HTT ... Read more